Dcode universal mutation detection system manual

V, universal mutation detection system, for 16 cm gels with twenty 1 mm wells, includes comb gasket, 2 sets of clamps, control reagents. The DCode system is a vertical electrophoresis instrument for the detection of gene mutations. The DCode system can be used to perform any vertical gel-based mutation detection method. The system is optimized for DGGE, CDGE, TTGE, SSCP, PTT and Heteroduplex Analysis. Some of the advantages of the DCode system include uniform buffer temperature around the gel, buffer circulation, buffer File Size: KB. The DCode universal mutation detection system can scan for single-base changes by any of the following electrophoresis techniques: Q Single-stranded conformation polymorphism (SSCP) analysis Q Denaturing gradient gel electrophoresis (DGGE) Q Constant denaturing gel electrophoresis (CDGE) Q Temporal temperature gradient gel electrophoresis (TTGE).

DGGE analysis of the amplified bacterial 16S rRNA gene was performed on the DCode Universal Mutation Detection System (Bio-Rad, USA) according to the instruction manual. The polyacrylamide gels (7% w/v) containing a linear formamide/urea gradient ranging from 25% to 65% denaturant were used. The gels were run for 6 h at V and 60 °C and. DGGE was performed with the DCode Universal Mutation Detection System (Bio-Rad Laboratories, Hercules, CA, USA). PCR products ( μ l) were applied directly onto 6% (w/v) polycrylamide gels in 1X TAE buffer (20 m m Tris-acetate [pH 7·4], 10 m m sodium acetate, 0·5 m m disodium EDTA) containing a linear denaturing gradient from 45 to 70%. dgge was performed using the dcode universal mutation detection system as described in the manu-facturer's manual (Bio-Rad, hercules, Ca, usa). approximately ng PCR products were loaded onto 8% (wt/vol) polyacrylamide gels in 1×Tae (40 mM Tris, 20 mM acetate, mM na 2-edTa) with denaturant-gradient of % (% denaturant was.

V, universal mutation detection system, for 16 cm gels with twenty 1 mm wells, includes comb gasket, 2 sets of clamps, control reagents. The Bio-Rad DCode Universal Mutation Detection System can scan for single-base changes by SSCP, DGGE, CDGE, or TTGE. At the center of the system is the temperature control module (which includes a microprocessor-controlled heater, a buffer-recirculating pump, and a stirrer). For techniques requiring accurate temperature control, such as SSCP and TTGE, the gels are immersed in the buffer, and temperatures are regulated between 5° and 70°C. Combine powerful electrophoresis techniques and sensitive detection methods to scan for single-base changes with the DCode universal mutation detection system.